Stem Cell Facility
We specialize in generating feeder-free induced pluripotent stem cell (iPSC) lines from human blood, skin fibroblasts, and other potential cell types. Our services include CRISPR gene editing, differentiation, and extensive cell culture expertise for a variety of human and animal cell lines. We also offer cell line expansion and storage services.
iPSC Reprogramming
Current iPSC Approach
We utilize the CytoTune-iPS 2.0 Sendai Reprogramming Kit (ThermoFisher Scientific) for iPSC generation.
Advantages
- Efficient reprogramming of blood and other cell types.
- Scalable cell line generation with minimal hands-on time.
- Utilizes a non-integrating cytoplasmic RNA vector that clears rapidly from cells.
iPSC Characterization Services
- Immunostaining of Pluripotent Markers:
- Surface markers: TRA-1-60 and SSEA4.
- Nuclear markers: Nanog, Oct4, Sox2.
- Mycoplasma Testing:
PCR-based testing (Microsart RESEARCH Mycoplasma) is included as part of our continuous quality control for generating iPSC lines. - Pluripotency Validation:
We use the NanoString nCounter Stem Cell Characterization Panel to validate pluripotency. - Molecular Karyotyping:
We perform molecular karyotyping using the ThermoFisher KaryoStat Assay.
iPSC Differentiation Services
We offer differentiation into a variety of lineages, including:
- Cardiomyocytes
- Endothelial cells
- Neuronal progenitors
- Hematopoietic stem cell progenitors
- Additional lineages available upon request
CRISPR Gene Editing Services
We provide targeted deletions and insertions in any iPSC line using the Neon NxT Electroporation System.
Additional Services
- Single cell cloning in collaboration with James Marvin (Flow Cytometry Core).
- CRISPR/Cas9 design in collaboration with Crystal Davey (Mutation Generation & Detection Core).
Affiliated Labs
We collaborate with other core labs for additional iPSC characterization and resources:
- Flow Cytometry:
Contact James Marvin, jmarvin@cores.utah.edu, 801-585-7382.
Flow Cytometry Core - Molecular Karyotyping:
Contact Nieca Bronson, Nieca.Bronson@hci.utah.edu, 801-587-4406.
Biorepository and Molecular Pathology Core - RNA Sequencing:
Contact Brian Dalley, dalley@hci.utah.edu, 801-585-7192.
Microarray Facility and High-Throughput Genomics Core - DNA Sequencing:
Contact Derek Warner, dwarner@cores.utah.edu, 801-581-4736.
DNA Sequencing & Genomics Cores - Gene Editing:
Contact Crystal Davey, mutrus@genetics.utah.edu, 801-581-0662.
Mutation Generation & Detection Core
Jin Hwan Kim
Senior Lab Specialist
Phone: 801-587-9418
Email: jinhwan.kim@biochem.utah.edu
Kameron Burger
Lab Specialist
Phone: 801-587-9418
Email: kami.burger@hsc.utah.edu
Zhenyu Yang
Lab Technician
Phone: 801-587-9418
Email: zhenyu.yang@hsc.utah.edu
Main Office
26 N Medical Drive, SLC, UT 84112
Wintrobe, Rm. 203
Monday–Friday: 8am–5pm
801-581-5889
DNA Extraction Facility
Wintrobe, Rm. 213
Monday–Friday: 8am–5pm
801-585-3849
Stem Cell Facility
Wintrobe, Rm. 208
Monday–Friday: 8am-5pm
801-587-9418