The CTRC has extensive experience in extracting high-quality gDNA from human blood. In addition to blood, we specialize in nucleic acid extraction from a wide range of tissues and cell types. We offer both manual and automated DNA and RNA extraction from human clinical or animal model biospecimens.
Additional Services
- Isolation of PBMCs, serum, plasma, cell pellets, and free-circulating DNA from whole blood.
- Expertise in isolating cell-type specific populations from blood and tissue.
- Cell culture experiments, including the generation of Epstein-Barr Virus (EBV)-transformed cell lines and the cultivation of fibroblasts from skin biopsies.
If you have special requests and are wondering whether our core can assist with processing your cells or tissues, please contact us. We may be able to perform the assay you have in mind.
Automated Nucleic Acid Isolation
We use the Kingfisher Duo sample purification system: Kingfisher Duo System
Advantages
- Magnetic bead-based isolation system.
- Suitable for any sample type.
- DNA & RNA isolation from FFPE tissue for cancer research.
- Automated purification of cfDNA and cfTNA from liquid biopsies.
- Sequential DNA & RNA isolation.
- Automated CTC isolation from whole blood.
- Exosome and extracellular vesicle isolation from liquid biopsies.
- Automated gDNA isolation for genomics applications.
- Automated RNA isolation for RNA-based therapeutics.
- Nucleic acid isolation from microbes.
- Nucleic acid purification for pathogen detection.
Manual Nucleic Acid Isolation Kits
We offer flexibility with any type of kit from any company. Our team can accommodate specific kit requests, but we primarily use the following kits:
- Whole Blood: Qiagen Flexigene DNA Kit
- Cells & Tissue: Qiagen QIAamp Mini DNA Kit
- Saliva: Qiagen QIAamp Maxi DNA Kit
- Whole Blood in PAXgene Collection Tubes: PAXgene Blood DNA Kit | PAXgene Blood RNA Kit
- Whole Blood in Tempus Collection Tubes: Tempus Spin RNA Isolation Kit
- RNA Purification from Cells & Tissue: RNeasy Kits
- Simultaneous RNA & DNA Isolation: Qiagen AllPrep DNA/RNA Kits
- Soil Extraction: DNeasy PowerSoil Pro Kits
- Methylation: EZ DNA Methylation Kit
qPCR Assays
We offer customized TaqMan assays performed on PCR in our Genomics Core (Applied Biosystems).
Quality Control of Nucleic Acids
The CTRC ensures nucleic acid sample quality and quantity using the following methods:
- Spectrophotometry: ThermoFisher Scientific Nanodrop One
- Fluorometric Quantitation: ThermoFisher Scientific Qubit 3
- Standard Gel Electrophoresis
- Automated Electrophoresis: Agilent 2200 TapeStation (housed in the High-throughput Genomics Core)
Daniel Winder
Senior Lab Specialist
Phone: 801-585-3849
Email: daniel.winder@utah.edu
Jessi Poulson
DNA Lab Technician
Phone: 801-585-3849
Email: jessi.poulson@utah.edu
Jake Davis
Lab Technician
Phone: 801-585-3849
Email: jake.davis@hsc.utah.edu
Erin Bridges
Lab Technician
Phone: 801-585-3849
Email: erin.bridges@hsc.utah.edu
Main Office
26 N Medical Drive, SLC, UT 84112
Wintrobe, Rm. 203
Monday–Friday: 8am–5pm
801-581-5889
DNA Extraction Facility
Wintrobe, Rm. 213
Monday–Friday: 8am–5pm
801-585-3849
Stem Cell Facility
Wintrobe, Rm. 208
Monday–Friday: 8am-5pm
801-587-9418