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Nucleic Acid Isolation (DNA and RNA Services)

Service Current Prices
DNA extraction blood $31.96
DNA extraction saliva $32.92
DNA extraction mouthwash $32.92
DNA extraction buffy coat $32.92
DNA extraction cheek tissue $32.92
DNA extraction soft tissue/other $32.92
Free-circulating DNA $38.22
DNA extraction cell culture lines (single well of 6 plate or smaller) $19.80
RNA extraction using PAXgene Blood RNA Kit (up to 8ml per sample) $41.98
RNA extraction soft tissue/other $22.06
RNA extraction cell culture lines (single well of 6-well plate or smaller) $22.06
Rnase Treatment per 8mls of blood $1.27
Qubit measurements per sample $2.23
Each Extra Aliquot $0.85

Blood and Cell Culture Services

Service Current Prices
EBV transformed cell line $92.54
Regrow cryopreserved EBV transformed cell lines $83.08
Serum or plasma isolation $7.80
PBMC isolation $31.73

Biorepository Service

Service Current Prices
1 cryobox stored int he -80 Freezer/Per month $0.52
1 cryobox stored in the Liquid Nitrogen/Per month $2.08

Translational Consultation Service

Service Current Prices
Per 30 minutes (Colin Maguire-Director) $38.22

CRISPR-iPSC Generation

Service Current Prices
2 separate gRNAs TBD
Electroporation of the CRISPRs into the iPSC line of interest TBD
TIDE analysis TBD
Flow sorting of iPSC clones TBD
Expansion TBD
Sequencing of clones to identify mutants TBD

Induced Pluripotent Stem Cell Services

iPSC generation per single patient sample (6-12 distinct cell lines) Standard Characterization is included in package

Service Current Prices
Supplies & Reagents for iPSC generation $577.59
Labor for iPSC generation $1,115.39
Cell line authentication Standard Characterization $59.80
Pluripotent Immunofluorescence Analysis Standard Characterization $257.83
Molecular Karyotyping Standard Characterization $356.50
Mycoplasma & Bacteria Testing Standard Characterization $99.39
Total $2,466.50

DNA for cell line authentication per patient

Service Current Prices
DNA extraction by CTRC $19.80
Cell line authentication by Genomics Core $40.00
Total $59.80

Immunofluorescence analysis: 3 nuclear pluripotent markers and 2 pluripotent surface markers per patient sample

Service Current Prices
Reagents & supplies for immunostaining and Imaging $116.60
Labor for imaging and analysis $131.22
Cell imaging core fee per hour (EVOS FL) $10.00
Total $257.83

TaqMan qPCR analysis: 4 blood markers, 4 pluripotent markers, and 2 reference genes per patient sample

Service Current Prices
RNA extraction by CTRC $22.06
qPCR reagent & supplies (See TaqMan qPCR Reagents Tab Catalog #'s $132.26
Genomics core qPCR instrument price per 384-well plate ($40 per plate [8 samples per plate]) $5.00
Labor for cDNA sysnthesis, qPCR, run, and analysis $240.58
Total $399.89

Molecular Karyotyping per patient sample

Service Current Prices
KaryoStat service provided by ThermoFisher Scientific $349.00
Shipping cost $7.50
Total $356.50

Mycoplasma & bacteria testing per patient sample

Service Current Prices
DNA extraction by CTRC (if DNA has not previously been extracted) $19.80
e-Myco Plus Mycoplasma PCR Detection Kit per patient sample (Bulldog Bio, Cat#25234, 96 tests per kit) (See attached ThermoFisher quote.  Quotes flucuate from month to month) $4.61
Bacterial Test Kit per patient sample (PromoKine, Cat# PK-CA91-2024, ThermoFisher Cat#50 310 61924, 48 tests per kit)(See attached ThermoFisher quote. Quotes flucuate from month to month). $9.36
Labor $65.61
Total $99.39

STEMdiff Trilineage Differentiation Analysis per patient sample

Service Current Prices
Reagents & supplies for immunostaining and Imaging $116.60
Labor for imaging and analysis $131.22
Cell imaging core fee per hour (EVOS FL) $10.00
Total $257.83

Cardiomyocyte Differentiation Analysis per patient sample

Service Current Prices
Reagents & supplies for immunostaining and Imaging $97.65
Labor for imaging and analysis $131.22
Cell imaging core fee per hour (EVOS FL) $10.00
Total $238.88

Neural Induction Analysis per patient sample

Service Current Prices
Reagents & supplies for immunostaining and Imaging $104.59
Labor for imaging and analysis $131.22
Cell imaging core fee per hour (EVOS FL) $10.00
Total $245.82